Dr.Tsoulfas received his MD from Brown University School of Medicine and completed a general surgery residency at the University of Iowa Hospitals and Clinics, as well as a transplant research fellowship at the Starzl Transplant Institute at the University of Pittsburgh. He then completed a two-year transplantation surgery fellowship at the Massachusetts General Hospital, Harvard Medical School and then joined the Division of Solid Organ Transplantation and Hepatobiliary Surgery at the University of Rochester Medical Center as an Assistant Professor of Surgery. He has currently moved back to Greece, where he is Professor of Transplantation Surgery and Chief at the Department of Transplantation Surgery at the Aristotle University of Thessaloniki School of Medicine and Director of the Center for Research and Innovation in Solid Organ Transplantation.  He has published over 230 papers in peer-reviewed Journals and in PubMed, as well as 46 book chapters with an H-index 38 and an i10-index 94 and 8460 citations.  He has edited 17 books and is a reviewer for 40 International Journal and on the Editorial Board of several others, including International Surgery and Annals of Surgical Oncology.

The recipient of awards such as the Edward E. Mason award for excellence in patient care and education, he is a member of a number of professional organizations including the Society of Surgical Oncology, the TTS, the American Society of Transplant Surgeons, Association for Academic Surgery, International College of Surgeons, American College of Surgeons, International Liver Transplantation Society, Society for Laparoscopic Surgeons and International HepaticoPancreaticoBilliary Association.  Also the recipient of the American College of Surgeons International Guest Scholarship. He has served as a member of multiple committees, including the International Relations Committee of the American College of Surgeons and the International Relations Committee of the American HepaticoPancreaticoBiliary Association (AHPBA).  Currently the president of the Greek Chapter of the International College of Surgeons, he has served as World President of the International College of Surgeons and Chair of the International Relations Committee of the American College of Surgeons. He has served as a member of the AHPBA and the IHPBA Education and Training Committees, the ILTS Education and Scholarship Committee and the E-AHPBA Education Committee (Training Program Accreditation), the ASTS CME and Professional Development committee and the AASLD Training and Workforce Committee and the Association of Surgical Education International Committee.  He is also a member of the Executive Council of the Hellenic Surgical Society and President of the Hellenic Transplantation Society. He was elected Vice-President of the Aristotle University School of Medicine in 2025 and Governor of the ACS Greek Chapter in October 2025.

Clinical and research interests include hepatobiliary surgery, primary and secondary hepatic malignancies, ischemia/reperfusion injury, solid organ transplantation, as well as medical/surgical education and the use of technology, including applications of artificial technology and 3D printing in surgery.